大鼠和兔颈动脉再狭窄模型比较
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Comparison of Rat and Rabbit Carotid Restenosis Model
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    目的为选择更理想的再狭窄动物模型,对大鼠和兔颈动脉再狭窄模型的组织病理变化进行比较。方法采用球囊导管损伤法剥脱大鼠和兔的一侧颈总动脉内膜,术后一周取损伤血管进行病理组织切片和HE染色。结果兔颈总动脉球囊损伤后的主要变化是平滑肌细胞迁移和血液细胞粘附,平滑肌细胞增殖尚不明显;而大鼠球囊损伤后的主要变化是血管壁细胞大量增殖,αactin免疫组织化学分析显示增殖的细胞为平滑肌细胞。结论研究抑制再狭窄发生机制中平滑肌细胞增殖时,大鼠颈总动脉球囊损伤动物模型更合适。

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    Aim Restenosis after percutaneous transluminal coronary angioplasty was one of the main clinic problems at present. A successful restenosis animal model was essential for its solution. So the rat and rabbit carotid restenosis models were built and compared to select more practical ones. Methods Balloon catheter was used to injure one of rats’ and rabbits’carotids. One week later the treated carotids were cutted, sliced and HE stained. Rat carotid well cells were cultured in vitro and analysed with α-actin immunohistochemistry. Results The main histopathologic changes of rabbit carotids after balloon injury were smooth muscle transfer and blood cells adheration. While the main change of rat’s was cell proliferation,and α-actin immunohistochemistry analysis confirmed the cell was smooth muscle cell. Conclusions Rat restenosis model was more practical for study of smooth muscle cell proliferation associated diseases,such as restenosis after percutaneous transluminal coronary angioplasty.

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苏海霞,盛净.大鼠和兔颈动脉再狭窄模型比较[J].中国动脉硬化杂志,2005,13(3):332~334.

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  • 收稿日期:2004-08-31
  • 最后修改日期:2005-01-17
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