Aim To explore the role of advanced glycation end products (AGEs)in pathogenesis and development of diabetic complications, and analyse the immunological property of monoclonal antibodies (McAb)against AGEs. Methods Indirect ELISA and western blotting were used to analyse the immunological property of McAb and the epitope which would combine with McAb. AGEs in serum of human and aortas from diabetic rats were also detected by McAb. Results McAb reacted with AGEs specially and combined with non-carboxymethyl lysine (non-CML). AGEs in serum of human and aortas from diabetic rats had been detected, however, there had been no AGEs detected in aortas from normal rats. Conclusion McAb(non-CML)reacted with AGEs specially,and might be of value for AGEs measurement.