Aim To observe the expression of angiotensinⅡ-induced cyclooxygenase-2 (COX-2) in cultured human umbilical vascular smooth muscle cells and investigate the influence of aspirin and fluvastatin on it. Methods Vascular smooth muscle cells were prepared from human umbilical arteries. The expression of COX-2 were observed in these cells after stimulated with different concentrations of angiotensinⅡ (AngⅡ) for 1 h, or stimulated with AngⅡ after treated with different concentrations of aspirin or fluvastatin or their combination for 1 h. Reverse transcription-polymerase chain reaction (RT-PCR) and cell immunocytochemistry were used for COX-2 mRNA and protein analysis. Results The expression of COX-2 mRNA was markedly increased under the stimulation of AngⅡ in a dose-dependent manner and reached to the peak when the concentration of AngⅡ was 1.0 μmol/L. This effect was inhibited by aspirin and fluvastatin, and the amount of COX-2 mRNA decreased gradually when the drugs dose increased. The amount of COX-2 mRNA was even lower in the drugs combination group than those in monotherapies groups (p<0.05). Results of the COX-2 protein expression with cell immunocytochemistry were coincident with those of mRNA expression with RT-PCR. Conclusions COX-2 can be induced by AngⅡ in cultured human vascular smooth muscle cells, which may play an important role in the progression of atherogenesis. Aspirin and fluvastatin can co-suppress the expression of COX-2, which may bring a novel therapeutic idea for drugs use.