Aim To investigate whether five lipoxygenase activating protein(FLAP) is involved in the mechanism of human umbilical vein endothelial cells(hUVEC) oxidation injury induced by oxidized low density lipoprotein(ox-LDL). Methods Isolated and cultured hUVEC were used as experimental model.hUVEC were induced by ox-LDL at 10,50,100 and 200 mg/L for 24 hours.Leukotriene C4(LTC4) levels in the cell supernatant fluid were detected with enzyme-linked immunosorbent assay(ELISA),cell viability was detected by MTT assay,and FLAP mRNA and FLAP protein were detected with fluorescent quantitation PCR and Western blot. Results Ox-LDL at 100 and 200 mg/L significantly induced the release of LTC4(P<0.01),the decrease of cell viability in hUVEC(P<0.01) and upregulation of FLAP mRNA expression and FLAP protein expression.The regressive analysis showed that the amounts of released LTC4 had significantly positive correlation with the concentration of ox-LDL(r=0.953,P<0.01). Conclusion FLAP could be involved in the LTC4 secretion and oxidative injury induced by ox-LDL in hUVEC.