Aim To investigate the effect of ARL-1 expression on acrolein induced oxidative stress in 293T cells. Methods EGFP/ARL-1 fusion protein was expressed in 293T cells by introducing EGFP/ARL-1 plasmid;the reactive oxygen species(ROS) were probed by CM-H2DCFDA and determined by FACS. Results The 293T show a fluorescence density of 530%±36% when treated with 10μmol/l of acrolein,whereas a fluorescence density of 220%±20% was detected in 293T cells with the expression of EGFP/ARL-1 under the same concentration of acrolein. Conclusion ARL-1 expression can decrease cellular ROS level and reduce acrolein induced oxidative stress.