Aim To observe the clinical effect of monosialotetrahexosylganglioside in treatment of ischemic stroke. Methods 106 cases of ischemic stroke were randomly divided into two groups,experimental group and control group,53 cases in each group.BI score were applied to assess the activities of daily living and level of awareness after a course of treatment to determine the efficacy.And serum S100β protein level changes were monitored before and after treatment. Results After one course of treatment,the total effective rate in experimental treatment group was 92.5% and 81.1% in the control group.BI score of the treatment group was significantly higher than that of the control group at 7th,14th and 21th days,and the differences were statistically significant(P<0.05).Compared with the control group,the experimental group achieved better nurological function after treatment,and the difference was statistically significant(P<0.05).The serum S100β protein levels in the treatment group decreased gradually at the 7th,14th and 21th days after treatment,significantly lower than the concentration of the same group before treatment,but also significantly lower than that of the control group.The differences were statistically significant(P<0.05). Conclusion Monosialotetrahexosylganglioside was effective in treatment of ischemic stroke,and without side effect,and deserved clinical application.