AimTo study the correlation between the cytochrome P450 (CYP) 3A5 gene polymorphism and dyslipidemia.MethodsThe real-time PCR genotyping at CYP3A5*3 (6986 A＞G) position was established using Taqman minor groove binding (MGB) probes.Total 321 dyslipidemia patients and 258 matched controls of Chinese Han population were genotyped at CYP3A5*3 (6986 A＞G) position using this method.Plasma lipid levels were measured by using routine method.ResultsAll three genotypes, AA, GA and GG existed in both dyslipidemia patients and control group.AA, GA, GG genotyped frequencies were 9.0%, 46.1%, 44.9% for patients and 9.7%, 39.5%, 50.8% for control group respectively; A，G allele frequencies were 32.1%, 67.9% for patients and 29.5%, 70.5% for control group respectively, in which the statistic significance was not observed （P>0.05）.But the results demonstrated that the presence of A allele was associated with high density lipoprotein cholesterol (HDLC) in patients group.ConclusionCYP3A5*3 (6986 A＞G) polymorphism was not associated with dyslipidemia in Chinese population, however, this polymorphism has some influence on serum HDLC level.