AimTo explore the role of advanced glycation end products (AGE) in pathogenesis and development of diabetic complications.MethodsIndirect ELISA and Western blotting were used to analyse the immunological property of McAb and the epitope which would be combined with McAb.AGE in serum of human and aortas, renal, heart from diabetic rats were also detected by McAb.ResultsMcAb reacted with AGE specially and was combined with non-carboxymethyl lysine (non-CML).AGE in renal and heart of diabetic rats had been detected, but were not obviously detected in normal rats.ConclusionMcAb（non-CML）reacted with AGE specially, and might be of value for AGE measurement.