Aim To study the effect of ligustrazine on hypertrophy of cardiomyocytes induced by angiotensin Ⅱ(AngⅡ). Methods The cultured cardiomyocytes were treated with AngⅡ to induce hypertrophy and given with ligustrazine of different densities.The cell size,3H-Leucine incorporation and the mRNA levels of β-myosin heavy chain(β-MHC) were examined. Results Stimulated by AngⅡ,the cardiomyocytes were enlarged under the contrast phase microscope.After stimulated by AngⅡ,3H-Leucine incorporation and the mRNA levels of β-MHC increased in the hypertrophic cardiocytes.Given by ligustrazine,these data decreased accordingly as the ligustrazine densities increased. Conclusion AngⅡ may induce the cardiomyocytes hypertrophy.Ligustrazine P could partly inhibit the cardiomyocytes hypertrophy induced by AngⅡ.