Aim To explore effects of hypoxia-reoxygenation on glucose oxidation and fatty acid oxidation(FAO) of hypertrophied cardiomyocytes. Methods Cultured rat cardiomyocytes were induced to be hypertrophy by angiotention Ⅱ(AngⅡ) and norepinephrine(NE),which was confirmed by -Leu incorporation in cardiomyocytes and detection surface area of cells.We established a model of post-hypoxia reoxygenation of cultured cardic cells in vitro.Glucose oxidation,FAO and acitivity of pyruvate dehydrogenase(PDH) and carnitine palmitoyltransferase(CPT) were determined by liquid scintillation counting. Results Surface area of cells increased by 63.94% and -Leu incorporation by 181.54%,when 0.1 μmol/L Ang Ⅱand 1 μmol/L NE were added in vitro.Activity of PDH decreased,and glucose oxidation decreased by 48%;Activity of PDH and glucose oxidation recovered to the level of prehypoxia at 8 hours of reoxygenation.Activity of CPT decreased,and FAO decreased by 60%;Activity of FAO and CPT recovered gradually to the level of prehypoxia during reoxygenation. Conclusion Glucose oxidation and fatty acid oxidation of hypertrophied cardiomyocyte decreased during hypoxia,and both recovered during reoxygenation.Activity of PDH and CPT was involved in these changes.