Aim To screen differential microRNA(miRNA) expression profiles of CD4+T lymphocyte from the patients with unstable angina pectoris(UAP) and the healthy controls by microarray analysis technique.To elucidate the mechanism responsible for modulation of CD4+T lymphocyte and provide insights into the effects of miRNA on UAP. Methods The CD4+T lymphocyte were isolated from mononuclear cells prepared with Ficoll-Hypaque density-gradients centrifugation from human peripheral blood by magnetic cell sorting system(MACS).The miRNA expressions profiles of CD4+T lymphocyte were screened using oligonucleotide microarray and differentially expressed miRNA were identified by using microarray analysis software.The microarray results were confirmed by real-time quantitative polymerase chain reaction(qRT-PCR). Results The expression of miR-155,miR-21,miR-424 and miR-127-3p were up-regulated over 1.5 folds,and the expression of miR-30b and miR-181a were down-regulated over 0.5 folds. The qRT-PCR results were in accordance with the microarray outcomes. Conclusion The differentially expressed miRNA of CD4+T lymphocyte may participate in the occurring and developing of UAP.