AimEstablishing model of cerebral ischemia-reperfusion injury and intervening with Danhong injection, then observing morphological changes of cerebral cell in damaged area and Golgi, detecting Golgi Matrix Protein 130 (GM130) expression, and exploring protective mechanism of Danhong injection on cerebral ischemia-reperfusion injury in rats.Methods72 healthy adult SD rats were randomly divided into normal controlled group (6), sham operation group (6), ischemia-reperfusion group (30), and Danhong intervention group (30).According to the reperfusion time after ischemia 2 h, sham operation group and ischemia-reperfusion group were respectively divided into 6 h, 24 h, 48 h, 72 h, and 7 days five subgroups, there were 6 rats in each subgroup.After the success of cerebral ischemia-reperfusion model, the rats in Danhong intervention group were interved with Danhong injection(8ml/kg,QD) by intraperitoneal injection from the recovery of reperfusion until they were executed; At the same time, the rats in ischemia-reperfusion group were treated with the same dose of normal saline by intraperitoneal injection.ResultsMorphology showed cortical neuronal survival number were significantly increased in Danhong intervention 7 d group compared with the other groups, and the damage degree was the lightest; In ischemia-reperfusion injury 7 d group, Golgi morphology took significantly impaired change, lack of network structure, lightly staining, granules decreasing or disappearing, and even some broken, but in Danhong intervention 7 d group, Golgi morphology kept normal.GM130 expression decreased with the prolongation of ischemia-reperfusion time, increased with the prolongation of Danhong injection intervention time.GM130 expression of Danhong intervention 7 d group was the highest compared with the other groups（p<0.05).ConclusionDanhong injection may maintain the Golgi stability through the up-regulation of GM130 expression, and thus play a neuroprotective role.