AimTo investigate the proliferation effects of overexpression integrin-linked kinase on human umbilical vein endotheial cells (HUVEC).MethodsHUVEC were infected by integrin-linked kinase (ILK) Ad-virus and induced ILK overexpression.HUVEC proliferation levels were measured by thiazolyl blue (MTT), flow cytometry and 5-ethynyl-2’-deoxyuridine (EDU) assay.Western blot was used to assess protein kinase B (Akt), endothelial nitric oxide synthase (eNOS) expression and phosphorylation levels.ResultsMTT assay, flow cytometry cycle, EDU assay all showed that cells proliferation were significantly higher in ILK overexpressed group than in the control group (p<0.01 or p<0.05).The levels of Akt and eNOS phosphorylation levels were also significantly higher than that in control group (p<0.05), but Akt and eNOS expression levels had no difference between the two groups.ConclusionsILK overexpression can promote HUVECs proliferation.This effect may be mediated through actvation of Akt and eNOS.