Aim To investigate the association of adiponectin promoter single nucleotide polymorphisms with metabolic syndrome in Hans of the northern Guangxi. Methods A total of 108 MS subjects were selected as MS group, and 76 normal adults were selected as control group. Measurement of height, weight, blood pressure, fasting blood glucose（FPG）, triglyceride （TG）,total cholesterol（TC）,low density lipoprotein cholesterol（LDLC）,high density lipoprotein cholesterol （HDLC）,uric acid（UA） and ultrasonic examination were performed and these clinical datas were compared between the two groups. The attributing factors of MS were analyzed by Logistic regression model. The polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was applied to analyze the differences of adiponectin gene promoter -11377C/G in the genotype and allele frequency between cases and controls. Many anthropometric and biological indices were compared between cases and controls in different genotype carriers. Results There was significant difference of clinical data（body mass index,biood pressure,FPG,TG,TC,LDLC, HDLC and UA） between MS and control groups（P<0.01 or P<0.05）. The multivariable stepwise analysis extracted sex,age,UA and fatty liver as major risk factors for MS（P<0.01 or P<0.05）. There was no significant difference of the distribution of the genotype and allele frequencies between MS and control groups. The anthropometric and biological indices were not significant among different genotypes in MS and control groups. Conclusions The adiponectin gene promoter -11377 is not associated with metabolic syndrome in Hans of the northern Guangxi.