Aim To study the change characteristics of vascular endothelial cell function and blood vessels regulating factor in different time of cerebral ischemia of rat model under the condition of hyperlipidemia, and analyse the effect of the factors of hyperlipidemia on vascular endothelial cells and blood vessel regulating factors. Methods Experimental rats were divided into normal control, normal sham operation group, day 3, day 7 after normal ischemia and hyperlipid, sham operation group, day 3, day 7 after ischemia with hyperlipidemia. High fat diets can successfully induce hyperlipidemia model. The ischemic model was established with line embolism to block the middle cerebral artery occlusion（MCAO）. The content of von Willebrand factor, NO, ET-1, 6-keto-FGF1α and TXB2 in serum were detected by ELISA. Results Compared with normal control and sham operation group, the content of vWF, and TXB2 in serum were increased at different time points of cerebral ischemia model. vWF was increased in day 7 after normal cerebral ischemia and in day 3 in hyperlipidemia concurrent cerebral ischemia group, with statistical significance （P<0.05）. And the content of ET-1 had no significant difference among groups and interclass （P>0.05）. TXB2 was also increased with statistical significance in day 3 and day 7 of simple cerebral ischemia （P<0.05）, but had no obvious change in hyperlipidemia concurrent cerebral ischemia （P>0.05）. NO and 6-keto-FGF1α were also increased with significant difference in in day 3 and day 7 of cerebral ischemia groups （P<0.05）. Compared with simple cerebral ischemia group, in hyperlipidemia concurrent cerebral ischemia group, vWF were increased in day 3 and in 7 days with significant difference （P<0.05）, ET-1 had no significant change（P>0.05）. However, TXB2 was decreased between day 3 and day 7 after ischemia with significant difference （P<0.05）. NO and 6-keto-FGF1α were also descreased, there was significant difference in day 7 （P<0.05）, but not in day 3 after ischemia （P>0.05）. Conclusion Under the condition of hyperlipidemia, blood vessels regulating factors have not only accumulated phenomenon, but also specific expression in the difference of cerebral ischemia period, which are contributed to the recovery of the lesion in recovery phase.