Aim To develop mouse model of diabetic atherosclerosis. Methods 46 male 8-week-old apolipoprotein E knockout （ApoE^-/-） mice were randomly divided into control group, high-fat group, streptozotocin （STZ） low-dose group and high-dose group, low-dose group was treated with 5 daily intraperitoneal injections of STZ 55 mg/kg per day, high-dose group was treated with intraperitoneal injection of STZ 200 mg/kg for once. After 2 months, all of the mice were sacrificed and the blood samples were collected, the body weight, fasting blood glucose （FBG）, total cholesterol （TG）, triglyceride （TC）, low density lipoprotein （LDL）, high density lipoprotein （HDL）, the plaque area of aortic root were detected. Results High-dose group had unacceptable mortality, low-dose group got higher FBG, TG, TC, LDL, HDL and more plaque area compared with control group and high-fat group （P＜0.01）, high-fat group got mildly elevated FGB （7.78±0.67 mmol/L）. Conclusions ApoE^-/- mice treated with 5 daily intraperitoneal injections of STZ is an ideal mouse model of diabetic atherosclerosis, ApoE^-/- mice supplied with high-fat diet got elevated fasting glucose, and ApoE^-/- mice treated with high-dose of STZ is not proper for the model.