Aim To investigate the protective effect of 5,7-Dihydroxyl-8-nitrio chrysin （8-NOChR） on the growth of vascular endothelial cell damaged by lysophosphatidyl choline （LPC） and its possible molecular mechanism. Methods Human umbilical vein endothelial cells （HUVEC） were incubated in vitro, the damaged effect of LPC on HUVEC cells and the rivalry effect of various concentration of 8-NOChR on HUVEC cells induced by LPC were evaluated by MTT assay. The colony formations were detected by plate colony formation assay. Distribution of cell cycle was analyzed by flow cytometry. Western blot assay was used to measure proteins related to cell cycle. Results MTT showed the viability of HUVEC treated with LPC decreased in a dose and time dependent manner compared with the cells treated with NS group and 0.1%DMSO group （P<0.05）, while 8-NOChR could increase the viability and the colony formations of cells treated with LPC in a dose and time-dependent manner compared with the LPC group （P<0.05）. FCM indicated the G₂/M accumulation of HUVEC treated with LPC combining 8-NOChR decreased in dose-dependent, and the expression of cyclin A and cyclin B protein were activated （P<0.05）, and protein level of P53 and P21 were down-regulated （P<0.05）, while the expression of CDK1 protein showed no change with the same treatment（P>0.05）. Conclusion 8-NOChR could significantly prevent the damaged HUVEC induced by LPC, which correlated with activation of cyclin A and cyclin B protein and down-regulation of P53 and P21 protein.