Aim To detect the anti-oxidation efficiency of vitamin E in different low density lipoprotein （LDL） oxidation models and explore the possible reasons for low antioxidant efficiency of vitamin E. Methods The active LDL oxidation model and passive LDL oxidation model were established in CLR-1730 cell line respectively. After the co-stimulation with LDL and VE for 0 h, 12 h, and co-stimulation with LPS and VE for 0 h,3 h, the mRNA and protein expression levels of human alpha-defensin-1（HNP-1）were detected by using real time PCR and ELISA. We set up 3 different VE working groups: VE added before the oxidation model（pre-VE）,vitamin E added after oxidation model（post-VE） and the control［VE（－）］. We detected malondialde （MDA）, protein carbonyl （PCO） and super oxide dlsmutase （SOD） to determine whether vitamin E interfere with the oxidation model. The level of oxygen free radicals in cells were observed by using flow cytometry and fluorescence microscopy . Results （1） The mRNA levels and protein levels of HNP-1 were increased in oxidation models （P<0.05）. （2） PCO detection among the three groups in active model showed no significant difference （P>0.05） while in passive model the pre-VE and post-VE results were significantly lower than the VE（－） （P<0.05）. PCO detection: the difference during pre-VE and post-VE in active model was not obvious, but both were significantly lower than the VE（－） （P<0.05）. SOD detection: pre-VE in active model was significantly higher than post-VE and VE（－）（P<0.05）. （3） In both oxidation models, the intracellular oxygen free radicals in the pre-VE and post-VE groups were higher than the control. Conclusion VE would not affect the establish-ment of active and passive oxidation models. The antioxidant efficiency of anti-lipid and protein oxidation is significantly different, while the activity of SOD were enhanced in both models.