Aim To investigate the relationship between severity of coronary heart disease （CHD） and plasma level of high-mobility group box1 protein（HMGB1）, high sensitive C-reactive protein（hs-CRP ）. Methods The study enrolled 100 patients suspected of CHD. All patients underwent coronary artery angiography. The patients were divided into CHD group and control group. The CHD group was divided into several subgroups according to the type of disease, Gensini score and the number of diseased coronary arteries. Serum HMGB1, hs-CRP, renal/hepatic function, blood lipids, blood glucose, myocardial markers were measured before coronary artery angiography, and history of smoking, hypertension, diabetes mellitus were also asked. The plasma level of HMGB1 and hs-CRP were compared among all groups to explore the correlation between plasma level of HMGB1,hs-CRP and severity of CHD. All data were analyzed by SPSS 20.0 for windows. Results ①Serum HMGB1 and hs-CRP level of CHD group were higher than the control group（P<0.01）. Serum HMGB1 and hs-CRP level of AMI group were higher than the UAP group（P<0.05）.