Aim To observe relationship between high-sensitivity C-reactive protein （hs-CRP）,high mobility group box-1 protein （HMGB1） and intercellular adhesion molecule-1 （ICAM1） with GRACE score,and to clarify the value of hs-CRP,HMGB1 and ICAM1 in estimating the criticality of non-ST segment elevation acute coronary syndrome （NSTEACS） patients.Methods According to the GRACE score NSTEACS patients were divided into different groups: risk group （≤108 scores）,middle risk group （109～140 scores）,high risk group （＞140 scores）,and 20 volunteers negative in coronary angiography （CAG） were as the control group. The levels of hs-CRP,HMGB1,ICAM1 in each group were detected,and the indexes were compared among various groups. Relationship between the indexes and GRACE score was analyzed.Results The levels of hs-CRP,HMGB1,ICAM1 in four groups were significantly different from each other. At the same time,hs-CRP,HMGB1,ICAM1 have significantly positive relationship with GRACE score （r＝0.498,0.561,0.526）. Conclusion hs-CRP,HMGB1,ICAM1 have intimate connection with GRACE score,and have practical value in estimating the criticality of NSTEACS patients.