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胰岛素对大鼠平滑肌细胞表达MMP-9、TIMP-1及内皮细胞表达sES的影响
作者:
作者单位:

(南华大学附属第一医院心血管内科,湖南省衡阳市 421001)

作者简介:

葛晨亮,硕士研究生,E-mail为458590567@qq.com。

基金项目:

湖南省卫生厅科研基金(B2012-049)


Effect of Insulin on the Expression of MMP9 and TIMP-1 Released from Smooth Muscle Cells and E-selectin Released from Endothelial Cells in SD Rats
Author:
Affiliation:

Cardiovascular Internal Medicine, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China)

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    摘要:

    目的 探讨胰岛素对SD大鼠平滑肌细胞表达基质金属蛋白酶9(MMP-9)、组织抑制因子1(TIMP-1)以及内皮细胞表达可溶性E-选择素(sES)的影响。方法 原代培养SD大鼠颈内动脉平滑肌细胞及主动脉内皮细胞,用不同剂量的胰岛素作用后,以酶联免疫吸附实验(ELISA)分别测定上清液MMP-9、TIMP-1及sES的水平。结果 与对照组(无胰岛素)相比,胰岛素组MMP-9及sES水平显著增高,TIMP-1则显著降低(P＜0.05)。经Pearson相关分析显示,胰岛素浓度与MMP-9(r=0.815,P＜0.001)及sES(r=0.841,P＜0.001)呈正相关,与组织抑制因子1(r=-0.740,P＜0.001)呈负相关。结论 胰岛素促进SD大鼠平滑肌细胞MMP-9及内皮细胞sES表达,抑制平滑肌细胞TIMP-1表达,并呈剂量依赖性。

    Abstract:

    Aim To investigate the effect of insulin on expression of MMP-9,TIMP-1 and sES. Methods Primary cultured smooth muscle cells from the internal carotid artery and endothelial cells from the aorta of SD rats treated with different doses of insulin,MMP-9, TIMP-1 and sES released by cells into medium were quantified by ELISA. Results MMP-9 and SES levels were higher in insulin group compared with control group,while TIMP-1 was less than control group(P＜0.05). Significant positive correlation was observed between insulin and MMP-9(r=0.815,P＜0.001)as well as sES(r=0.841,P＜0.001), negative correlation was observed between insulin and TIMP-1(r=-0.740,P＜0.001). Conclusion Insulin augmented the secretion of SES and MMP-9, attenuated the secretion of TIMP-1 in dependence on the concentration.

    参考文献
    [1] Papazafiropoulou A, Tentolouris N.Matrix metalloproteinases and cardiovascular diseases .Hippokratia, 2009, 13(2):76-82.
    [2] Serra R, Grande R, Montemurro R, et al.The role of matrix metalloproteinases and neutrophil gelatinase-associated lipocalin in central and peripheral arterial aneurysms .Surgery, 2015, 157(1):155-162.
    [3] Dahi S, Karliner JS, Sarkar R, et al.Transgenic expression of matrix metalloproteinase-2 induces coronary artery ectasia .Int J Exp Pathol, 2011, 92(1):50-56.
    [4] Daoud EM, Abdelaziz AA, Hassan NA.Isolated coronary artery ectasia debate:Inflammation versus atherosclerosis .Egypt Heart J, 2012, 64(4):185-190.
    [5] Brunetti ND, Salvemini G, Cuculo A, et al.Coronary artery ectasia is related to coronary slow flow and inflammatory activation .Atherosclerosis, 2014, 233(2):636-640.
    [6] Adiloglu AK, Ocal A, Tas T, et al.Increased expression of CD11a and CD45 on leukocytes and decreased serum TNF-alpha levels in patients with isolated coronary artery ectasia .Clin Lab, 2011, 57(9-10):703-709.
    [7] Korematsu S, Ohta Y Fau - Tamai N, Tamai N Fau - Takeguchi M, et al.Cell distribution differences of matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 in patients with Kawasaki disease .Pediatr Infect Dis J, 2012, 31(9):973-974.
    [8] Zhang T, Xu J, Li D, et al.Salvianolic acid A, a matrix metalloproteinase-9 inhibitor of Salvia miltiorrhiza, attenuates aortic aneurysm formation in apolipoprotein E-deficient mice .Phytomedicine, 2014, 15(10):1 137-145.
    [9] Chung AW, Au Yeung K, Sandor GG, et al.Loss of elastic fiber integrity and reduction of vascular smooth muscle contraction resulting from the upregulated activities of matrix metalloproteinase-2 and -9 in the thoracic aortic aneurysm in Marfan syndrome .Circ Res, 2007, 101(5):512-522.
    [10] Ciavarella C, Alviano F Fau - Gallitto E, Gallitto E Fau - Ricci F, et al.Human Vascular Wall Mesenchymal Stromal Cells Contribute to Abdominal Aortic Aneurysm Pathogenesis Through an Impaired Immunomodulatory Activity and Increased Levels of Matrix Metalloproteinase-9 .Circ J, 2015, 79(7):1 460-469.
    [11] Zhang T, Xu J, Li D, et al.Salvianolic acid A, a matrix metalloproteinase-9 inhibitor of Salvia miltiorrhiza, attenuates aortic aneurysm formation in apolipoprotein E-deficient mice .Phytomedicine, 2014, 21(10):1 137-145.
    [12] Dogan A, Tuzun N Fau - Turker Y, Turker Y Fau - Akcay S, et al.Matrix metalloproteinases and inflammatory markers in coronary artery ectasia:their relationship to severity of coronary artery ectasia .Coron Artery Dis, 2008, 19(8):559-563.
    [13] Triantafyllidi H, Rizos I, Rallidis L, et al.Aortic distensibility associates with increased ascending thoracic aorta diameter and left ventricular diastolic dysfunction in patients with coronary artery ectasia .Heart Vessels, 2010, 25(3):187-194.
    [14] Murase Y, Yagi K, Kobayashi J, et al.Association of coronary artery ectasia with plasma insulin levels in Japanese men of heterozygous familial hypercholesterolemia with the low-density lipoprotein receptor gene mutation K790X .Clin Chim Acta, 2005, 355(1-2):33-39.
    [15] Duellman T, Warren Cl Fau - Peissig P, Peissig P Fau - Wynn M, et al.Matrix metalloproteinase-9 genotype as a potential genetic marker for abdominal aortic aneurysm .Circ Cardiovasc Genet, 2012, 5(5):529-537.
    [16] Roggerio A, Sambiase NV, Palomino SA, et al.Correlation of bacterial coinfection versus matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 expression in aortic aneurysm and atherosclerosis .Ann Vasc Surg, 2013, 27(7):964-971.
    [17] Dogan A, Tuzun N, Turker Y, et al.Matrix metalloproteinases and inflammatory markers in coronary artery ectasia:their relationship to severity of coronary artery ectasia .Coron Artery Dis, 2008, 19(8):559-563.
    [18] Triantafyllis AS, Kalogeropoulos As Fau - Rigopoulos AG, Rigopoulos Ag Fau - Sakadakis EA, et al.Coronary artery ectasia and inflammatory cytokines:link with a predominant Th-2 immune response? .Journal Article Cytokine, 2013, 64(1):427-432.
    [19] Fischoeder A, Meyborg H, Stibenz D, et al.Insulin augments matrix metalloproteinase-9 expression in monocytes .Cardiovasc Res, 2007, 73(4):841-848.
    [20] Kim JA, Montagnani M Fau - Koh KK, Koh Kk Fau - Quon MJ, et al.Reciprocal relationships between insulin resistance and endothelial dysfunction:molecular and pathophysiological mechanisms .Circulation, 2006, 113(15):1 888-904.
    [21] Armato J, DeFronzo RA, Abdul-Ghani M, et al.Successful treatment of prediabetes in clinical practice:targeting insulin resistance and beta-cell dysfunction .Endocr Pract, 2012, 18(3):342-350.
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葛晨亮,刘厂辉,唐振旺,吴洁,苏华,王刚,占凡,谢楠,陈仲.胰岛素对大鼠平滑肌细胞表达MMP-9、TIMP-1及内皮细胞表达sES的影响[J].中国动脉硬化杂志,2016,24(2):145~149.

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  • 收稿日期:2015-08-07
  • 最后修改日期:2015-11-10
  • 在线发布日期: 2016-06-30

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