Aim To study the effect of angelica on miR-122 in myocardial tissue of spontaneously hypertensive rats (SHR) and its bioinformatics analysis. Methods All the spontaneously hypertensive rats were divided into angelica group, model group, captopril group and angelica captopril group, the same-age Wistar rats as normal control group, then the systolic blood pressure of the tails of all rats in different groups were measured before and after treatments. After 4 weeks, myocardial tissue of the rats were extracted to test miRNA expression profiling. Results Angelica can reduce blood pressure levels in SHR. 13 miRNAs were found up-regulated and 16 miRNAs down-regulated in angelica group, and 15 miRNAs were found up-regulated and 13 miRNAs down-regulated in captopril group and 4 miRNAs were found up-regulated and 21 miRNAs down-regulated in angelica captopril group. We made a analysis of biological process of miR-122, which was up-regulated in all of the three groups, and an amino acid transport gene Slc7a1 was found. By miRNA target precdiction, 8 miRNAs were found targeted by Slc7a1 in angelica group and 11 miRNAs were found targeted by Slc7a1 in captopril group and 11 miRNAs were found targeted by Slc7a1 in captopril group. Conclusions Angelica can regulate the expression of miR-122, with the mechanism that angelica influenced endothelial function by the target gene Slc7a1 of miR-122 and resulted in regulation of blood pressure, which established a foundation of further research.