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二陈汤合桃红四物汤含药血清对ox-LDL诱导内皮细胞损伤的保护作用及机制
作者:
作者单位:

(1.广州中医药大学第一临床医学院,广东省广州市 510405;2.辽宁中医药大学中医脏象理论及 应用教育部重点实验室,辽宁省沈阳市 110847;3.广州中医药大学第一附属医院,广东省广州市 510405)

作者简介:

王俊岩,博士,研究方向为中医药防治心血管疾病,E-mail为junyan_wang@163.com。通信作者杨关林,教授,博士研究生导师,研究方向为中西医结合防治心脑血管疾病,E-mail为 yang_guanlin@163.com。通信作者冼绍祥,教授,博士研究生导师,研究方向为中医药防治心脑血管疾病,E-mail为zhongyfy@126.com。

基金项目:

国家自然科学基金项目(81774022、81300229)；国家重点基础研究发展计划项目(973计划)(2013CB531704)


Effect and mechanism of serum containing of Erchen decoction and Taohong Siwu decoction on ox-LDL induced endothelial cell damage
Author:
Affiliation:

1.The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China;2.Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Ministry of Education of China, Shenyang, Liaoning 110847, China;3.The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China)

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    摘要:

    目的 观察二陈汤合桃红四物汤含药血清对氧化型低密度脂蛋白(ox-LDL)诱导内皮细胞损伤的保护作用并探讨其可能的机制。方法 培养EA.hy926细胞,随机分为对照组、ox-LDL组、干预组、抑制剂组。流式细胞仪检测各组细胞凋亡水平,比色法检测细胞内丙二醛(MDA)、一氧化氮(NO)水平及超氧化物歧化酶(SOD)活性,ELISA检测细胞上清液中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)水平,Western blot检测磷脂酰肌醇3-激酶(PI3K)、磷酸化AKT(p-AKT)、内皮型一氧化氮合酶(eNOS)蛋白表达水平。结果 与对照组比较,ox-LDL组细胞上清液中TNF-α和IL-6水平显著升高(P＜0.01),细胞内MDA含量显著升高(P＜0.01),SOD活性明显降低(P＜0.05),上清液中NO水平显著降低(P＜0.01),活性氧(ROS)含量和细胞凋亡率明显升高,PI3K、eNOS蛋白表达水平和AKT蛋白磷酸化水平显著降低(P＜0.01)。与ox-LDL组比较,干预组细胞上清液中TNF-α和IL-6水平显著降低(P＜0.01),细胞内MDA含量显著降低(P＜0.01),SOD活性明显升高(P＜0.05),上清液中NO水平显著升高(P＜0.01),ROS含量和细胞凋亡率明显降低,PI3K、eNOS蛋白表达水平和AKT蛋白磷酸化水平显著升高(P＜0.01)。与干预组比较,抑制剂组细胞上清液中TNF-α和IL-6水平显著升高(P＜0.01),细胞内MDA含量显著升高(P＜0.01)、SOD活性明显降低(P＜0.05),上清液中NO水平明显降低(P＜0.05),细胞内ROS含量升高,PI3K、eNOS蛋白表达水平和AKT蛋白磷酸化水平显著降低(P＜0.01)。结论 二陈汤合桃红四物汤含药血清能有效保护ox-LDL诱导的内皮细胞损伤,其机制可能与激活PI3K/AKT/eNOS信号通路有关。

    Abstract:

    Aim To investigate the effect and mechanism of Erchen decoction and Taohong Siwu decoction containing serum on oxidized low density lipoprotein (ox-LDL) induced endothelial cell damage. Methods EA.hy926 cells were cultured in vitro, then randomly divided into control group, ox-LDL group, intervention group, inhibitor group.Cell apoptosis was detected by flow cytometry, malondialdehyde (MDA), nitric oxide (NO) and superoxide dismutase (SOD) activity were detected by colorimetry, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in cell supernatant were detected by ELISA, and phosphatidylinositol 3-kinase (PI3K), phosphorylated AKT (p-AKT) and endothelial nitric oxide synthase (eNOS) protein expressions were detected by Western blot. Results Compared with control group, the level of TNF-α and IL-6 increased significantly (P＜0.01), MDA content in cells increased significantly (P＜0.01), SOD activity decreased (P＜0.05), NO level in supernatant decreased significantly (P＜0.01), content of reactive oxygen species (ROS) and apoptosis rate increased significantly, the protein expression level of PI3K, eNOS and the protein phosphorylation of AKT were significantly decreased (P＜0.01) in ox-LDL group. Compared with ox-LDL group, the level of TNF-α and IL-6 in cell supernatant decreased significantly (P＜0.01), MDA content in cells decreased significantly (P＜0.01), SOD activity increased significantly (P＜0.05), NO level in the supernatant increased significantly (P＜0.01), ROS content and apoptosis rate decreased significantly, the protein expression level of PI3K, eNOS and the protein phosphorylation of AKT were significantly increased (P＜0.01) in intervention group. Compared with intervention group, the level of TNF-α and IL-6 increased significantly (P＜0.01), MDA content in cells increased significantly (P＜0.01), SOD activity decreased (P＜0.05), NO level in supernatant decreased significantly (P＜0.05), content of ROS and apoptosis rate increased significantly, the protein expression level of PI3K, eNOS and the protein phosphorylation of AKT were significantly decreased (P＜0.01) in inhibitor group. Conclusion The serum containing of Erchen decoction and Taohong Siwu decoction can effectively protect ox-LDL induced endothelial cell injury, which may be related to activation of PI3K/AKT/eNOS signaling pathway.

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引用本文

王俊岩,曲宁宁,贾连群,陈文娜,张林,宋囡,杨关林,冼绍祥.二陈汤合桃红四物汤含药血清对ox-LDL诱导内皮细胞损伤的保护作用及机制[J].中国动脉硬化杂志,2018,26(10):987~992.

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  • 收稿日期:2018-04-19
  • 最后修改日期:2018-05-17
  • 在线发布日期: 2018-11-09

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