基于微生物16S rDNA测序技术研究高脂血症豚鼠肠道菌群的变化
作者:
作者单位:

(1.天津市医药科学研究所,天津市300020;2.天津市滨海新区大港医院,天津市300270)

作者简介:

王宏,硕士,助理研究员,研究方向为心脑血管药理,E-mail为wh19860906@126.com。通信作者杨景明,主任医师,E-mail为yangjmdg@126.com。

基金项目:

天津市滨海新区卫生局科技项目(2014BWKL001)


The changes of gut microbiota of hyperlipemia guinea pigs by microbial 16S rDNA sequencing
Author:
Affiliation:

1. Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China;2. Tianjin Coastal New-region Dagang Hospital, Tianjin 300270, China)

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    摘要:

    目的 利用微生物16S rDNA测序技术研究高脂血症豚鼠肠道菌群的变化情况。方法 豚鼠随机分为对照组和模型组,每组10只。高脂喂养8周复制高脂血症模型。第8周末测定豚鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDLC)和高密度脂蛋白胆固醇(HDLC)水平;剖取肝脏、主动脉和结肠,HE染色观察肝脏、主动脉和结肠组织形态学变化;16S rDNA测序检测豚鼠肠道内容物微生物组成和比例的变化情况。结果 与对照组相比,模型组豚鼠血清TC、TG、LDLC和HDLC水平均显著升高(P<0.01);组织形态学显示,模型组豚鼠均出现重度脂肪肝病变,但仅1只模型组豚鼠出现少量泡沫细胞聚集。16S rDNA基因序列分析显示,与对照组相比,在门分类学水平上,模型组豚鼠肠道互养菌门细菌比例显著增加(P<0.05);在属分类学水平上,模型组豚鼠肠道毛螺旋菌NK4A136、瘤胃球菌、幽门螺杆菌、Odoribacter、Allobaculum和Caldicoprobacter细菌比例显著降低(P<0.05),依赖杆菌、毛螺旋菌XPB1014、锥形杆菌和Enterorhabdus细菌比例明显升高(P<0.05)。结论 高脂血症豚鼠肠道菌群的组成及比例发生了明显变化。该实验结果为基于肠道菌群研究高脂血症作用机制奠定了理论基础。

    Abstract:

    Aim To study the changes of gut microbiota of hyperlipemia guinea pigs by microbial 16S rDNA sequencing. Methods Guinea pigs were randomly divided into normal group and model group, with 10 in each group. Guinea pig models of hyperlipidemia were established by high fat diet. After feeding for 8 weeks, serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDLC) and high density lipoprotein cholesterol (HDLC) levels were detected. HE staining was performed to detect the histological changes of liver, aorta and colon. The changes of gut microbiota were detected by 16S rDNA analysis. Results The levels of TC, TG, LDLC and HDLC of model group were higher than that of control group (P<0.01). According to the histomorphological test, all guinea pigs in the model group showed severe fatty liver, but only one of them appeared early symptoms of atherosclerosis. The gut microbiota 16S rDNA high-throughput sequencing analysis result showed that, compared with control group, at the phyla level, the proportion of Synergistetes was obviously increased (P<0.05); at the genus level, the proportions of lachnospiraceae NK4A136, ruminococcus, helicobacter, odoribacter, allobaculum and caldicoprobacter of the model group were significantly reduced (P<0.05); the proportions of fretibacterium, lachnospiraceae XPB1014, pyramidobacter and enterorhabdus were obviously increased (P<0.05). Conclusions The compositions and proportions of gut microbiota in guinea pigs were significantly changed by high-fat feeding. The results of the experiment laid a theoretical foundation for the study of the mechanism of hyperlipidemia based on intestinal flora.

    参考文献
    [1] 胡秋峰, 张勇.血糖、血脂及肥胖因素与脂肪肝的关系研究.现代医药卫生, 6,2(3):422-423.
    [2] 孙少卫, 董文娟, 谢雪娇, 等.动脉粥样硬化的中西医观.中国动脉硬化杂志, 6,4(5):524-528.
    [3] 周筱琼, 于小妹, 叶雄伟, 等.高脂血症患者血浆致动脉粥样硬化指数与血栓形成危险因子的相关性.中国动脉硬化杂志, 0,8(10):799-802.
    [4] 马武男.社区居民高脂血症与饮食和生活方式的关系研究.临床医药文献电子杂志, 7,4(18):3544-3545.
    [5] 李超, 崔立红.高脂血症、高脂饮食与肠道菌群的关系.世界华人消化杂志, 3,1(14):1273-1277.
    [6] 朱玲军, 项美香.双歧杆菌三联活菌胶囊联合阿托伐他汀片对高脂血症患者血脂、载脂蛋白及肠道菌群水平的影响.中国微生态学杂志, 6,8(10):1175-7,4.
    [7] 熊静芳, 傅国胜.高脂血症患者肠道优势菌群与血清脂质水平相关性研究.中国微生态学杂志, 3,5(11):1282-1285.
    [8] Xiangdong L, Yuanwu L, Hua Z, et al.Animal models for the atherosclerosis research:a review.Protein Cell, 1,2(3):189-201.
    [9] 李金莲.豚鼠高脂血症、早期动脉粥样硬化模型的建立、机理探讨及与大鼠模型的比较研究.中国协和医科大学, 2009.
    [10] 孔路科.高密度脂蛋白亚类在临床相关疾病中的分布特征.国际检验医学杂志, 4,5(7):878-881.
    [11] Tian L, Fu M.The relationship between high density lipoprotein subclass profile and plasma lipids concentrations.Lipids Health Dis, 0,9:118.
    [12] 徐燕华, 傅明德, 刘秉文, 等.不同类型高脂血症患者血清高密度脂蛋白亚类组成的研究.临床心血管病杂志, 3,9(2):83-86.
    [13] Zhang Y, Si Y, Yao S, et al.Celastrus orbiculatus Thunb.decreases athero-susceptibility in lipoproteins and the aorta of guinea pigs fed high fat diet.Lipids, 3,8(6):619-631.
    [14] Ye P, Cheah IK, Halliwell B.High fat diets and pathology in the guinea pig.Atherosclerosis or liver damage?.Biochim Biophys Acta, 3,2(2):355-364.
    [15] Wu ZX, Li SF, Chen H, et al.The changes of gut microbiota after acute myocardial infarction in rats.PLoS One, 7,2(7):e0180717.
    [16] Lin CH, Chen YH, Tsai TY, et al.Effects of deep sea water and Lactobacillus paracasei subsp.paracasei NTU 101 on hypercholesterolemia hamsters gut microbiota.Appl Microbiol Biotechnol, 7,1(1):321-329.
    [17] Everard A, Lazarevic V, Gaa N, et al.Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity .ISME J, 4,8(10):2116-2130.
    [18] Ravussin Y, Koren O, Spor A, et al.Responses of gut microbiota to diet composition and weight loss in lean and obese mice .Obesity(Silver Spring), 2,0(4):738-747.
    [19] Chen D, Yang Z, Chen X, et al.The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model .BMC Complement Altern Med, 4,4:386-394.
    [20] Shao Y, Hou D, Peng Q, et al.Lactobacillus plantarum HNU082-derived improvements in the intestinal microbiome prevent the development of hyperlipidaemia .Food Funct, 7,8(12):4508-4516.
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王宏,江茜,康利,袁玲,刘洪斌,王蕾,杨景明.基于微生物16S rDNA测序技术研究高脂血症豚鼠肠道菌群的变化[J].中国动脉硬化杂志,2018,26(10):1011~1015.

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  • 收稿日期:2018-03-07
  • 最后修改日期:2018-06-29
  • 在线发布日期: 2018-11-09