冠状动脉粥样斑块内CD45表达水平与病灶结构变化的关系
作者:
作者单位:

(贵州医科大学法医学院,贵州省贵阳市 550004)

作者简介:

王玉,硕士研究生,研究方向为法医病理学,E-mail为1514972498qq.com。通信作者万昌武,教授,硕士研究生导师,研究方向为法医病理学,E-mail为2668503694qq.com。

基金项目:

贵州省现代法医司法鉴定工程研究中心项目(黔发改高技[2016]1345号);贵州省科技合作计划项目(黔科合LH字[2015]7320号);贵州省科技厅联合基金(黔科合LG字[2012]003号、黔科合LG字[2012]030号)


CD45 expression level in coronary atherosclerotic plaques and its relationship to lesion structure changes
Author:
Affiliation:

Department of Forensic Medicine, Guizhou Medical University, Guiyang, Guizhou 550004, China)

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    摘要:

    目的 通过检测冠状动脉粥样硬化病灶内CD45蛋白表达水平,分析其与病灶结构变化的关系,探讨CD45在人冠状动脉粥样硬化病灶发生发展中的作用。方法 收集贵州医科大学法医司法鉴定中心司法鉴定中尸体检验及组织学检查确认有冠状动脉粥样硬化病例的冠状动脉组织,根据冠状动脉病变情况将其分为对照组、单纯动脉粥样硬化组(单纯As组)和动脉粥样硬化并继发病变组(As并继发病变组),常规石蜡切片HE染色观察各组冠状动脉的组织结构,免疫组织化学染色法、蛋白免疫印迹法及实时荧光定量PCR检测CD45蛋白的表达分布及水平,分析CD45表达水平与粥样硬化病灶结构变化的关系。结果 与对照组比较,有动脉粥样硬化及并发继发病变的冠状动脉斑块内纤维帽厚度变薄,病灶厚度、坏死灶厚度及血管腔面积增加(P均<0.05)。与对照组比较,有动脉粥样硬化及并发继发病变的血管组织CD45蛋白表达显著升高(P<0.05),且As并继发病变组显著高于单纯As组(P<0.05)。与对照组比较,有动脉粥样硬化及发生继发病变的冠状动脉病灶内CD45的表达显著升高(P<0.05),且As并继发病变组显著高于单纯As组(P<0.05),CD45阳性蛋白表达主要分布于斑块肩部和底部的白细胞(棕黄色着色)。与对照组比较,有动脉粥样硬化及并发继发病变的血管组织CD45 mRNA表达显著升高(P<0.05),且As并继发病变组显著高于单纯As组(P<0.05)。病灶内CD45表达水平与病灶结构变化具有相关性。 结论 人冠状动脉粥样斑块内CD45分子作为炎症反应的标志物,其表达水平反映病灶内的炎症反应程度,而病灶内炎症反应程度能影响病灶结构,从而导致斑块稳定性改变。

    Abstract:

    Aim By examining the level of CD45 protein expression in coronary atherosclerotic lesions, and to analyze the relationship between CD45 and lesions structure changes and explore the role of CD45 in the development of coronary atherosclerotic lesions in humans. Methods Specimens of coronary arteries were obtained from Forensic Judicial Expertise Center, Guizhou Medcial University with coronary atherosclerosis. The coronary arteries were classified into three groups according to the lesions:control, atherosclerosis, atherosclerosis and secondary lesion. Routine HE staining of paraffin sections was performed to observe the histological structure of coronary artery in the three groups. The expression level of CD45 protein was detected by immunohistochemical staining, Western blot and Real-time PCR. The relationship between the expression of CD45 and the structural changes of atherosclerotic lesions were analyzed. Results (1)Compared with the control group, the thickness of the fibrous cap in the coronary arterial plaque with atherosclerosis and secondary lesions became thinner, and the thickness of the lesion, the thickness of the necrosis lesion, and the lumen area of the vessel increased (P<0.05). (2)There was a statistically significant difference in the expression of CD45 protein between vascular tissues. (3)There was a statistically significant difference in the expression of CD45 between the control group, the atherosclerosis group and the secondary lesions, and it was mainly expressed in the all leukocytes of the plaques shoulder and bottom. (4)There was a statistically significant difference in the expression of CD45 mRNA between three groups. (5) CD45 expression was related to lesion structure changes. Conclusion The level of CD45 expression reflects the extent of inflammation in human coronary atherosclerotic plaque, and the extent of inflammation within the lesion can affect the structure of the lesion.

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王玉,孙晓宇,罗亚,乐翠云,汪家文,黄江,王杰,夏冰,汪元河,李明杰,万昌武.冠状动脉粥样斑块内CD45表达水平与病灶结构变化的关系[J].中国动脉硬化杂志,2019,27(2):114~119, 140.

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  • 收稿日期:2018-06-28
  • 最后修改日期:2018-09-18
  • 在线发布日期: 2019-01-21