量子降脂仪对高脂血症大鼠血脂的影响及其机制
作者:
作者单位:

(1. 中国科学院苏州生物医学工程技术研究所,江苏省苏州市215163;2.吉林省农业科学院农产品加工研究所,吉林省长春市130033)

作者简介:

孙德春,硕士,工程师,研究方向为生物医学工程,E-mail为dechunsun@xianyingkeji.com。通信作者李盛钰,博士,副研究员,研究方向为发酵食品及功能性益生菌,E-mail为lisy720@126.com。

基金项目:

吉林省科技厅项目(201603050YY,20160312019X);长春市科技局项目(16SS10)


Effect and mechanism of quantum lipid-lowering instrument on blood lipid in rats with hyperlipidemia
Author:
Affiliation:

1.Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, China;2.Institute of Agro-food Technology, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China)

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    摘要:

    目的 观察量子降脂仪对高脂血症模型大鼠的降血脂作用并探讨其机制。方法 以高脂饲料和10%果糖自由饮水建立无特定病原体动物(SPF)级大鼠高脂血症模型。量子降脂仪输出功率强度为70 μW/(cm2·min),每天治疗1~2次,治疗时间为每次5~10 min。采用Elisa试剂盒检测血清中血脂水平,Western blot方法检测脂类代谢和炎症通路关键蛋白表达水平的变化。结果 量子降脂仪明显改善由高脂血症引起的的血脂代谢紊乱、调节血脂平衡、降低炎症状态、缓解肝功能损伤、缓解氧化损伤、提高脂肪代谢相关酶的活性、抑制脂肪酸合成酶的活性。Western blot结果显示,量子降脂仪通过调节核因子κB(NF-κB)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)蛋白的表达水平,缓解机体慢性炎症;通过调节过氧化体增殖物激活型受体α(PPARα)蛋白的表达水平促进机体脂质代谢;通过调节胆固醇调节元件结合蛋白2(SREBP-2)通路,促进低密度脂蛋白受体(LDLR)蛋白表达,缓解脂质代谢紊乱,调节机体高胆固醇和高甘油三酯水平。结论 量子降脂仪具有明显降血脂作用,可作为降血脂医疗器械辅助应用于临床。

    Abstract:

    Aim To investigate the effects and mechanisms of quantum lipid-lowering instrument on lipid regulation. Methods High fat diet and 10% fructose were used to establish a rat model of hyperlipidemia. The output power of the quantum lipid-lowering instrument was 70 μW/cm2, and the treatment time was 5~10 minutes for each time,1~2 times a day. Elisa kit was used to detect serum lipid levels and Western blot was used to measure the expressions of key protein in lipid metabolic and inflammatory pathways. Results Quantum lipid-lowering instrument can improve the disorder of blood lipid metabolism, regulate blood lipid balance, reduce inflammation, alleviate liver function damage, alleviate oxidative damage, increase the activity of lipid metabolism-related enzymes, and inhibit the activity of fatty acid synthase which is caused by hyperlipidemia. Western blot showed that Quantum lipid-lowering instrument could alleviate inflammation by regulating the expression levels of nuclear factor κB (NF-κB), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6);modulate the expression of peroxisome proliferators-activated receptors α (PPARα) which could improve lipid metabolism;regulate the sterol-regulatory element binding proteins 2 (SREBP-2) pathway to increase low-density lipoprotein receptor (LDLR) protein expression, alleviate lipid metabolism disorders, and regulate cholesterol and triglyceride levels. Conclusion Quantum lipid-lowering instrument has obvious effect of lipid regulation, and can be used as an assisted clinical instrument for lowering lipid.

    参考文献
    [1] Navar-Boggan AM, Peterson ED, D'Agostino RBS, et al.Hyperlipidemia in early adulthood increases long-term risk of coronary heart disease.Circulation, 5,1(5):451-458.
    [2] 杨玮春, 胡敏予.高脂血症与脑损伤、脑神经递质的研究进展.中国动脉硬化杂志, 7,5(9):948-952.
    [3] Roumeguère T, Wespes E, Carpentier Y, et al.Erectile dysfunction is associated with a high prevalence of hyperlipidemia and coronary heart disease risk.Eur Urol, 3,4(3):355-359.
    [4] Ipsen DH, Lykkesfeldt J, Tveden-Nyborg P.Molecular mechanisms of hepatic lipid accumulation in non-alcoholic fatty liver disease.Cell Mol Life Sci, 8,5(18):3313-3327.
    [5] 杨诗杰, 苏汝好, 项岚, 等.降血脂药的应用现状及研究进展.医药论坛杂志, 6,7(1):90-92.
    [6] 叶明翔, 裴建明.他汀类药物的功与过:降血脂药的使用新理念.心脏杂志, 2010, (3):433-436.
    [7] 孙德春, 张国龙, 侯旭辉, 等.量子降脂仪的安全性评价.中国药物与临床, 7,7(11):1591-1595.
    [8] Rooyen DMV, Farrell GC.SREBP-2:a link between insulin resistance, hepatic cholesterol, and inflammation in NASH.J Gastroenterol Hepatol, 1,6(5):789-792.
    [9] Miyazaki M, Ntambi JM.Role of stearoyl-coenzyme a desaturase in lipid metabolism.Curr Opin Lipidol, 8,8(2):113-121.
    [10] Chen M, Zhang J, Sampieri K, et al.An aberrant SREBP-dependent lipogenic program promotes metastatic prostate cancer.Nat Genet, 8,0(2):206-218.
    [11] Yang TT, Koo MW.Chinese green tea lowers cholesterol level through an increase in fecal lipid excretion.Life Sci, 9,6(5):411-423.
    [12] 冷雪, 贾连群, 杨关林, 等.化瘀祛痰方对脾虚型高脂血症大鼠肝脏SREBP-2信号通路的干预作用.中国中西医结合杂志, 5,5(3):320-326.
    [13] Lozano I, Werf RV, Bietiger W, et al.High-fructose and high-fat diet-induced disorders in rats:impact on diabetes risk, hepatic and vascular complications.Nutr Metab, 6,3(1):1-13.
    [14] Li W, Lu Y.Hepatoprotective effects of sophoricoside against fructose-induced liver injury via regulating lipid metabolism, oxidation, and inflammation in mice.J Food Sci, 8,3(2):552-558.
    [15] Jeong HJ, Koo HN, Na HJ, et al.Inhibition of TNF-α and IL-6 production by aucubin through blockade of NF-κB activation RBL-2H3 mast cells.Cytokine, 2,8(5):252-259.
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孙德春,赵子健,高磊,孙健,杨舸,李晓文,李盛钰.量子降脂仪对高脂血症大鼠血脂的影响及其机制[J].中国动脉硬化杂志,2019,27(3):211~215.

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  • 收稿日期:2018-09-20
  • 最后修改日期:2018-12-10
  • 在线发布日期: 2019-01-28