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基于UPLC/LTQ-Orbitrap-MS技术对去卵巢大鼠的脂质组学研究
作者:
作者单位:

(南京中医药大学附属医院 江苏省中医院妇科, 江苏省南京市 210029)

作者简介:

何若晗,硕士研究生,研究方向为绝经综合征的基础与临床,E-mail为592222057@qq.com。通信作者卢苏,主任中医师,教授,博士研究生导师,研究方向为绝经综合征的基础和临床,E-mail为lusu0926@sina.com。

基金项目:

国家自然科学基金资助项目(81574009)；江苏省自然科学基金资助项目(BK20151600)；江苏省卫生计生委科研课题资助项目(Q201602)


Lipidomics study of ovariectomized rats based on UPLC/LTQ-Orbitrap-MS technology
Author:
Affiliation:

Department of Gynaecology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Jiangsu, Nanjing 210029, China)

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    摘要:

    目的 分析绝经期大鼠脂代谢物的变化,进而识别代谢变化中潜在的生物标志物。方法 将4月龄雌性SD大鼠随机分为假手术(Sham)组和去卵巢(OVX)组。采用UPLC/TQ-O rbitrap-MS的代谢组学分析方法,用主成分分析法(PCA)以及正交-偏最小二乘法分析法(OPLS-DA)进行多元统计分析,筛选与疾病有关的潜在生物标记物。结果 通过OPLS-DA得分图能够将假手术组和去卵巢组的大鼠血浆脂质代谢物很好地聚类区分,两组之间无明显的交叉重叠。与假手术组相比,去卵巢组中神经酰胺(Cer)、鞘磷脂(SM)、血小板活化因子(PAF)、溶血磷脂酰胆碱(LPC)、甘油三酯(TG)、ω-6不饱和脂肪酸(ω-6PUFA)的相对含量上升；甘油二酯(DG)、磷脂酰丝氨酸(PS)和磷脂酰甘油(PG)的相对含量下降。两组共测得35种内源性代谢产物,其中Cer、SM、PAF、LPC、TG、DG、PS、PG、ω-6PUFA等9种为潜在的生物标记物。结论 筛选出的9种潜在标志物均与绝经综合征有关,为老年性退化性疾病生物标志物的早期发现奠定基础。

    Abstract:

    Aim To analyze the changes of lipid metabolites in menopausal rats and identify potential biomarkers in metabolic changes. Methods 4-month-old female SD rats were randomly divided into sham operation (Sham) group and ovariectomized (OVX) group. Using UPLC/LTQ-Orbitrap-MS metabonomics analysis method, principal component analysis (PCA) and orthogonal-partial least squares analysis (OPLS-DA) were used for multivariate statistical analysis to screen potential biomarkers related to diseases. Results The OPLS-DA score plot could be used to distinguish the plasma lipid metabolites of rats in the sham group and ovariectomized group, and there was no significant overlap between the two groups. Compared with the sham group, the relative contents of ceramide (Cer), sphingomyelin (SM), platelet activating factor (PAF), lysophosphatidylcholine (LPC), triglyceride (TG) and omega-6 unsaturated fatty acid (ω-6PUFA) increased in the ovariectomized group, while the relative contents of diglyceride (DG), phosphatidylglycerin (PG) and phosphatidylserine (PS) decreased. A total of 35 endogenous metabolites were detected in both groups, including Cer, SM, PAF, LPC, TG, DG, PS, PG, ω-6PUFA as potential biomarkers. Conclusion All the 9 potential biomarkers were related to menopausal syndrome, which laid a foundation for the early detection of biomarkers of senile degenerative diseases.

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何若晗,陈赟,卢苏.基于UPLC/LTQ-Orbitrap-MS技术对去卵巢大鼠的脂质组学研究[J].中国动脉硬化杂志,2019,27(4):323~329.

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  • 收稿日期:2018-10-15
  • 最后修改日期:2019-01-11
  • 在线发布日期: 2019-04-08

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