Aim To investigate the role of interlekutin-10 (IL-10) in cardiomyocyte metabolism. MethodsCardiomyocyte lipotoxicity and glucose utilization reduction was induced by palmitate. Cardiomyocytes were treated with IL-10 and 2-NBDG uptake assay was adopted to assess the glucose utilization in cells. Lactic acid and oxidative phosphorylation were used to assess glycolysis and oxidation of glucose, respectively. Immuno-fluorescence was used to evaluate mitophagy in cardiomyocytes. RT-qPCR and Western blot were used to detect PINK1 expression. Results Compared with the control, IL-10 treatment approximately doubled the amount of 2-NBDG in cardiomyocytes. The supernatant and intracellular lactic acid were both reduced. Moreover, LC3-GFP was enhanced in mitochondrial aggregation and the PINK1 expression was up-regulated, which indicated mitophagy increase in cardiomyocytes. Conclusion IL-10 may re-balance cardiomyocytes metabolism substrates by increasing glucose utilization, glycolysis and oxidative phosphorylation through inducing mitophagy mediated by PINK1 up-expression.