Aim To investigate the effect of Xuezhikang (XZK) on apoptosis induced by oxidized low density lipoprotein (ox-LDL) and its possible mechanism. Methods Human umbilical vein endothelial cells (HUVEC) were cultured and divided into three groups, namely control group, ox-LDL group and ox-LDL+XZK(different dosages)groups.Cell counting kit-8 was used to test cell survival, Annexin V-FITC/PI apoptosis detection kit was used to count apoptotic rate of HUVEC among different groups, reactive oxygen species (ROS) were detected by ROS assay kit, expression of cytochrome C (CytC), caspase-3 and PARP-1 were analyzed by Western blot. Results XZK (25 mg/L, 50 mg/L and 100 mg/L) showed an antagonistic effect on apoptosis and ROS production of HUVEC induced by ox-LDL, and 100 mg/L XZK downregulated the protein expression of CytC, caspase-3 and PARP-1. Conclusion XZK reduces ROS production, leading to less expression of CytC, caspase-3 and PARP-1, thus prevents mitochondrial apoptotic signaling from activating, and eventually protects HUVEC from apoptosis.