2025年4月11日 周五
首页 > 按月查看>2020年第9月 >744-748
PDF 导出
抑制糖原合酶激酶活性改善动脉粥样硬化小鼠内皮祖细胞功能
作者:
作者单位:

(第三军医大学新桥医院全军心血管内科中心,重庆市 400037)

作者简介:

崔斌,博士,副主任医师,研究方向为血管内皮损伤的防治,E-mail为cuibin_xqhospital@126.com。通信作者黄岚,博士,主任医师,研究方向为冠心病诊断和治疗,E-mail为huanglan260@126.com。

基金项目:

国家自然科学基金资助项目(81100149)


Inhibition of glycogen synthase kinase 3β improves the proliferation, migration and adhesion of endothelial progenitor cells in atherosclerotic mice
Author:
Affiliation:

Institute of Cardiovascular Disease of PLA, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China)

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献
    • |
  • 相似文献
    • |
  • 引证文献
    • |
    摘要:

    目的 探讨抑制糖原合酶激酶3β(GSK3β)活性对动脉粥样硬化(As)小鼠内皮祖细胞(EPC)数量、增殖、迁移及黏附功能的影响。方法 建立As小鼠模型,密度梯度离心法分离培养As小鼠骨髓源EPC,基因转染法转染抑制GSK3β活性的重组缺陷型腺病毒(基因转染组)至对数生长期的EPC。采用镜下计数法、MTT法、改良Boyden小室迁移试验及黏附试验法测定GSK3β活性对As小鼠EPC数量、增殖、迁移及黏附能力的影响。结果 与正常对照组比较,As小鼠EPC数量显著减少,细胞增殖、迁移及黏附能力明显受损(P＜0.01,n＝5)。与As组比较,抑制GSK3β活性的基因转染组EPC数量(P＜0.01,n＝5)及增殖能力(P＜0.05,n＝5)显著增加,EPC的迁移功能及黏附功能均明显改善(P＜0.01,n＝5)。结论 抑制GSK3β活性可增加As小鼠EPC数量并显著改善其增殖、迁移及黏附功能。

    Abstract:

    Aim To investigate the effect of glycogen synthase kinase 3β(GSK3β)inhibition on the proliferation, migration and adhesion of endothelial progenitor cells(EPC)in atherosclerotic mice. Methods Atherosclerotic mice model were established. Mononuclear cells were isolated and cultured from bone marrow in atherosclerotic mice (atherosclerotic group) and wild type mice (normal control group). EPC in logarithmic phase were transduced with replication defective adenovirus vector expressing catalytically inactive glycogen synthase kinase 3β(GKS3β-KM)(gene transfer group) in atherosclerotic mice. The number and biological functions of EPC, such as proliferation, migration and adhesion were assessed by cells count, MTT assay, modified Boyden's chamber migrationtrial and adhesion test. Results Compared with normal control group, the number of EPC was observably reduced and proliferation, migration and adhesion capacities of EPC were markedly impaired in atherosclerotic mice(P＜0.01,n＝5). Compared with atherosclerotic group, the number (P＜0.01,n＝5)and biological functions of EPC including proliferation (P＜0.01,n＝5), migration (P＜0.01,n＝5) and adhesion were significantly enhanced in gene transfer group (P＜0.01,n＝5). Conclusion GSK3β inhibition could improve impaired proliferation, migration and adhesion capabilities of EPC in atherosclerotic mice.

    参考文献
    [1] Leal V, Ribeiro CF, Oliveiros B, et al.Intrinsic vascular repair by endothelial progenitor cells in acute coronary syndromes:an update overview.Stem Cell Rev Rep, 9,5(1):35-47.
    [2] Yang JX, Pan YY, Wang XX, et al.Endothelial progenitor cells in age-related vascular remodeling .Cell Transplant, 8,7(5):786-795.
    [3] Wils J, Favre J, Bellien J.Modulating putative endothelial progenitor cells for the treatment of endothelial dysfunction and cardiovascular complications in diabetes .Pharmacol Ther, 7,0:98-115.
    [4] Luo S, Xia W, Chen C, et al.Endothelial progenitor cells and hypertension:current concepts and future implications.Clin Sci (Lond), 6,0(22):2029-2042.
    [5] 方叶青, 谢培益, 王磊, 等.冠心病患者循环内皮祖细胞变化及功能的研究.中国动脉硬化杂志, 9,7(8):694-699.
    [6] 崔斌, 刘曦, 李佳蓓, 等.氯化锂通过激活Wnt信号通路促进内皮祖细胞的增殖能力.中国动脉硬化杂志, 7,5(10):985-988.
    [7] Cui B, Huang L, Fang YQ, et al.Transplantation of endothelial progenitor cells overexpressing endothelial nitricoxide synthase enhances inhibition of neointimal hyperplasia and restores endothelium-dependent vasodilatation .Microvasc Res, 1,1(1):143-150.
    [8] 崔斌, 黄岚, 武晓静, 等.内皮祖细胞移植对血管内膜修复的影响.中国病理生理杂志, 7,3(4):625-628.
    [9] 崔斌, 刘曦, 秦浙学, 等.抑制糖原合酶激酶活性对老龄大鼠内皮祖细胞增殖的作用机制.西部医学, 8,0(10):1426-1433.
    [10] Ruan C, Shen Y, Chen R, et al.Endothelial progenitor cells and atherosclerosis.Front Biosci (Landmark Ed), 3,8:1194-1201.
    [11] Kong M, Zhao Y, Chen A, et al.The importance of physiologic ischemia training in preventing the development of atherosclerosis:the role of endothelial progenitor cells in atherosclerotic rabbits .Coron Artery Dis, 9,0(5):377-383.
    [12] 陈丽, 王凤娇, 薛雅卓, 等.颈动脉粥样硬化影响内皮祖细胞功能的研究进展.中国动脉硬化杂志, 8,6(4):419-423.
    [13] Tu C, Xu R, Koleti M, et al.Glycogen synthase kinase-3 inhibition sensitizes human induced pluripotent stem cells to thiol-containing antioxidants induced apoptosis.Stem Cell Res, 7,3:182-187.
    [14] Yoda K, Ohnuki Y, Kurosawa H.Optimization of the treatment conditions with glycogen synthase kinase-3 inhibitor towards enhancing the proliferation of human induced pluripotent stem cells while maintaining an undifferentiated state under feeder-free conditions.J Biosci Bioeng, 9,7(3):381-387.
    [15] Gao L, Zhao M, Ye W, et al.Inhibition of glycogen synthase kinase-3 (GSK3) promotes the neural differentiation of full-term amniotic fluid-derived stem cells towards neural progenitor cells.Tissue Cell, 6,8(4):312-320.
    [16] Hibbert B, Ma X, Pourdjabbar A, et al.Inhibition of endothelial progenitor cell glycogen synthase kinase-3beta results in attenuated neointima formation and enhanced re-endothelialization after arterial injury .Cardiovasc Res, 9,3(1):16-23.
    [17] Ma X, Hibbert B, Dhaliwal B, et al.Delayed re-endothelialization with rapamycin-coated stents is rescued by the addition of a glycogen synthase kinase-3beta inhibitor .Cardiovasc Res, 0,6(2):338-345.
    引证文献
引用本文

崔斌,于世勇,赵晓辉,李佳蓓,秦浙学,喻杨,黄岚.抑制糖原合酶激酶活性改善动脉粥样硬化小鼠内皮祖细胞功能[J].中国动脉硬化杂志,2020,28(9):744~748.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2019-10-28
  • 最后修改日期:2019-12-09
  • 在线发布日期: 2020-10-28

您是本站第 4765961 访问者

邮编：421001 传真：0734-8160523

电话：0734-8160765 E-mail：dmzzbjb@163.net

中国动脉硬化杂志 ® 2025 网站版权所有