藏红花素对急性心肌梗死大鼠心肌线粒体的保护作用
作者:
作者单位:

(邯郸矿业集团有限公司总医院,河北省邯郸市 056105)

作者简介:

范雷娇,主治医师,研究方向为心血管内科疾病,E-mail为xiaomangguo1229@163.com

基金项目:

河北省医学科学研究课题计划(20191837)


Protective effect of Crocin on myocardial mitochondria in rats with acute myocardial infarction
Author:
Affiliation:

General Hospital of Handan Mining Group Co., Ltd., Handan, Hebei 056105, China)

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    摘要:

    目的 探讨藏红花素(Crocin)对急性心肌梗死(AMI)大鼠缺血区心肌线粒体损伤的保护作用及其相关机制。方法 将150只SD大鼠按随机数字表法分为假手术组、AMI组和藏红花素7.5、15、30 mg/kg组,各30只;通过结扎左冠状动脉前降支复制AMI大鼠模型,藏红花素各组于造模手术前10 min腹腔注射给药,假手术组和AMI组给予生理盐水。24 h后,通过透射电子显微镜观察缺血区心肌线粒体超微结构变化,通过荧光分光光度计检测膜电位、膜通透性转换孔(MPTP)开放度;氧电极法检测呼吸功能指标[态3呼吸(R3)、态4呼吸(R4)、呼吸控制率(RCR)],比色法检测呼吸酶活性、ATP含量,定磷法检测心肌Na+-K+-ATP酶、Ca2+-ATP酶活性,原子化学发光法测定线粒体Ca2+浓度。结果 与AMI组比较,藏红花素15、30 mg/kg组线粒体肿胀、膜破裂、嵴断裂溶解等超微结构病变明显改善,膜电位升高且MPTP开放度降低(P<0.01),R3、RCR升高且R4降低(P<0.05或P<0.01),线粒体呼吸酶(NADH脱氢酶、琥珀酸脱氢酶、细胞色素C氧化酶)活性、ATP含量且心肌Na+-K+-ATP酶、Ca2+-ATP酶活性升高(P<0.05或P<0.01),线粒体Ca2+浓度降低(P<0.05或P<0.01)。结论 藏红花素对AMI大鼠缺血区心肌线粒体结构和功能具有保护作用,作用机制可能与抑制线粒体Ca2+浓度升高有关。

    Abstract:

    Aim To investigate the protective effect of Crocin on myocardial mitochondria in rats with acute myocardial infarction (AMI) and its mechanism. Methods 150 SD rats were randomly divided into Sham group, AMI group, Crocin (7.5,5, 30 mg/kg) group according to random number table method (n=30). The AMI rat model was established by ligating the left anterior descending coronary artery. 24 h later, the ultrastructure changes of myocardial mitochondria in ischemic area was observed by transmission electron microscope; the membrane potential and the opening of mitochondrial permeablity transition pore (MPTP) were detected by fluorescence spectrophotometer; the myocardial mitochondrial respiratory function index (R3, R4, RCR) were detected by oxygen electrode method. The respiratory enzymes activity, ATP content were detected by colorimetry method; the Na+-K+-ATPase, Ca2+-ATPase activity were detected by phosphorus determination method; the mitochondrial Ca2+ concent was detected by atomic chemiluminescence. ResultsCompared with AMI group, the ultrastructural lesions such as mitochondrial swelling, membrane rupture, crest rupture and dissolution were significantly improved in Crocin 5,0 mg/kg groups, the membrane potential increased and MPTP openness decreased (P<0.01); the R3, RCR increased and R4 decreased (P<0.05 or P<0.01); respiratory enzymes (NADH dehydrogenase, succinate dehydrogenase, cytochrome C oxidase) activity, ATP content and Na+-K+- ATPase, Ca2+-ATPase activity increased (P<0.05 or P<0.01), Ca2+ concentration decreased (P<0.05 or P<0.01). Conclusion Crocin has protective effect on the structure and function of myocardial mitochondria in the ischemic area of AMI rats, which mechanism may be related to inhibiting the increase of mitochondrial Ca2+ concentration.

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引用本文

范雷娇,韩国卫.藏红花素对急性心肌梗死大鼠心肌线粒体的保护作用[J].中国动脉硬化杂志,2021,29(5):383~388.

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  • 收稿日期:2020-06-12
  • 最后修改日期:2020-07-26
  • 在线发布日期: 2021-04-23