Aim To explore the effect and possible mechanism of tirofiban on oxidized low density lipoprotein (ox-LDL) -induced injury of umbilical vein endothelial cells (EA.hy926). Methods Low, medium and high doses of tirofiban were applied to ox-LDL-induced EA.hy926, and cell viability and apoptosis were detected by cell counting kit (CCK-8) and flow cytometry, respectively. The expression of miR-22 was detected by real-time fluorescent quantitative PCR (qRT-PCR). Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) in cell culture supernatant. miR-22 mimics were transfected into EA.hy926 to up-regulate miR-22 expression, and the effect of up-regulating miR-22 on ox-LDL-induced EA.hy926 cell injury was detected by the above method. Results After tirofiban acted on ox-LDL-induced EA.hy926 cells, the cell survival rate was significantly increased, the apoptosis rate was significantly reduced, and the content of TNF-α and IL-6 in the cell culture supernatant was significantly reduced, the expression of miR-22 was significantly increased (P＜0.05). After up-regulating the expression of miR-22, the content of TNF-α and IL-6 in the culture supernatant of EA.hy926 cells induced by ox-LDL was significantly reduced, the cell survival rate was significantly increased, and the apoptosis rate was significantly reduced (P＜0.05). Conclusion Tirofiban could alleviate ox-LDL-induced apoptosis and inflammation of umbilical vein endothelial cells, and its mechanism may be related to the up-regulation of miR-22 expression.