Aim To explore the predictive value of plasma microRNA-93 (miR-93) level for in-stent restenosis (ISR) after interventional therapy in patients with lower extremity arteriosclerosis obliterans (LASO). Methods 184 patients with LASO admitted to the Affiliated Hospital of North China University of Technology from February 2016 to May 2019 were selected as the research objects. According to whether the patients with LASO had ISR within one year after intervention, they were divided into two groups:ISR group (n＝71) and non-ISR group (n＝113). Real-time fluorescence quantitative PCR was used to detect the plasma miR-93 level of LASO patients, and to explore the relationship between miR-93 and ISR. Results Comparing the plasma miR-93 levels between the ISR group and the non-ISR group, the differences between the two groups including time effect, inter-group effect, and time-to-group interaction effect were statistically significant (P＜0.01). The area under the receiver operating characteristic curve, sensitivity, and specificity of postoperative 14 days miR-93 in diagnosing ISR were 0.9,1.55%, and 70.80%, respectively, and its diagnostic efficiency for ISR was higher than those of preoperative miR-93 and postoperative 7 days miR-93, the differences were statistically significant (P＜0.001). Multivariate Logistic regression analysis showed that the length of vascular occlusion, postoperative 14 days miR-93, low density lipoprotein cholesterol and C-reactive protein were independent risk factors affecting ISR in LASO patients (P＜0.01). Restricted cubic spline fitting Logistic regression analysis showed that miR-93 was related to ISR in LASO patients (P＜0.001), and there was a non-linear relationship (P＝0.009). Conclusion Plasma miR-93 level in LASO patients is related to ISR, and the high level of plasma miR-93 at 14 days after interventional surgery suggests that LASO patients have a high risk of ISR within one year after operation.