2025年4月3日 周四
首页 > 按月查看>2023年第4月 >322-328. DOI:10.20039/j.cnki.10073949.2023.04.006
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牡荆苷上调FGD5-AS1抑制缺氧复氧诱导的心肌细胞氧化应激和凋亡
作者:
作者单位:

(1.成都市第三人民医院蒲江医院 心血管内科,四川省成都市 611630;2.成都市第三人民医院蒲江医院 心内科,四川省成都市 611630)

作者简介:

刘火军,主治医师,研究方向为高血压、心力衰竭和心律失常,E-mail:leasnw@163.com。通信作者左晓琴,副主任医师,研究方向为心肌病、心力衰竭和心律失常,E-mail:leasnw@163.com。

基金项目:

四川省卫生和计划生育委员会科研课题项目(18ZD007)


Vitexin inhibits oxidative stress and apoptosis of cardiomyocytes induced by hypoxia and reoxygenation by up-regulating FGD5-AS1
Author:
Affiliation:

1.Department of Cardiovascular Medicine, Chengdu, Sichuan 611630, China;2.Department of Cardiology, the Third People's Hospital of Chengdu Pujiang Hospital, Chengdu, Sichuan 611630, China)

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    摘要:

    目的]探究牡荆苷对缺氧复氧(H/R)诱导的心肌细胞损伤的影响及其与FGD5-AS1的调控关系。 [方法]大鼠心肌细胞H9c2分为对照组,H/R组,H/R+牡荆苷低、中、高剂量组,H/R+pcDNA组,H/R+pcDNA-FGD5-AS1组,H/R+牡荆苷+si-NC组和H/R+牡荆苷+si-FGD5-AS1组。流式细胞术检测细胞凋亡,Western blot检测Caspase-3、cleaved Caspase-3蛋白表达,试剂盒检测SOD活性和MDA含量,RT-PCR检测FGD5-AS1表达。 [结果]与H/R组比较,H/R+牡荆苷低、中、高剂量组凋亡率各降低13%、25%、48%,Caspase-3蛋白表达量各下降21%、38%、56%,cleaved Caspase-3蛋白表达各下降17%、40%、65%,MDA含量各下降15%、36%、52%,SOD活性升高0.88、2.73、3.86倍,FGD5-AS1表达各升高0.84、1.84、3.00倍(均P＜0.05),呈浓度依赖性。与H/R+pcDNA组比较,H/R+pcDNA-FGD5-AS1组凋亡率、Caspase-3和cleaved Caspase-3蛋白表达、MDA含量分别下降60%、70%、74%、60%(均P＜0.05),SOD活性升高4.04倍(P＜0.05)。与H/R+牡荆苷+si-NC组比较,H/R+牡荆苷+si-FGD5-AS1组凋亡率、Caspase-3和cleaved Caspase-3蛋白表达、MDA含量上升0.72、1.21、1.38、0.93倍(均P＜0.05),SOD活性降低67%(P＜0.05)。 [结论]牡荆苷可抑制H/R诱导的H9c2细胞氧化应激和凋亡,其作用机制可能与上调细胞中FGD5-AS1表达有关。

    Abstract:

    Aim To investigate the effect of vitexin on hypoxia/reoxygenation (H/R)-induced myocardial cell injury and its regulatory relationship with FGD5-AS1. Methods Rat cardiomyocytes H9c2 were divided into control group, H/R group, H/R+vitexin L, M,Hü group, H/R+pcDNA group, H/R+pcDNA-FGD5-AS1 group, H/R+vitexin+si-NC group and H/R+vitexin+si-FGD5-AS1 group. Cell apoptosis was detected by flow cytometry, Caspase-3 and cleaved Caspase-3 protein expressions were detected by Western blot, and the activity of SOD and the content of MDA in cells were detected by kits. The expression of FGD5-AS1 was detected by RT-PCR. Results Compared with H/R group, the apoptosis rate of H/R+vitexin L, M,Hü groups was decreased by 13%, 25% and 48%, respectively; Caspase-3 protein expression was decreased by 21%, 38% and 56%, respectively; Cleaved Caspase-3 protein expression was decreased by 17%, 40% and 65%, respectively; And MDA content was decreased by 15%, 36% and 52%(P＜0.05); But the activity of SOD was increased by 0.8,2.73 and 3.86 times, and the expression of FGD5-AS1 was increased by 0.4,1.84 and 3.00 times (P＜0.05). Compared with H/R+pcDNA group, the apoptosis rate, Caspase-3 and cleaved Caspase-3 protein expressions and MDA content were decreased in H/R+pcDNA-FGD5-AS1 group by 60%, 70%, 74%, and 60%, respectively (P＜0.05), but the activity of SOD was increased by 4.04 times (P＜0.05). Compared with the H/R+vitexin+si-NC group, the apoptosis rate of H9c2 cells, the expressions of Caspase-3 and cleaved Caspase-3 protein and the content of MDA were increased in the H/R+vitexin+si-FGD5-AS1 group by 0.2,1.1,1.8,0.93 times (P＜0.05), but the activity of SOD was decreased by 67%(P＜0.05). Conclusion Vitexin could inhibit H/R-induced apoptosis and oxidative stress of H9c2 cells, and its mechanism may be related to the up-regulation of FGD5-AS1 expression in cells.

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刘火军,左晓琴,杨萌卓,唐享强.牡荆苷上调FGD5-AS1抑制缺氧复氧诱导的心肌细胞氧化应激和凋亡[J].中国动脉硬化杂志,2023,31(4):322~328.

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  • 收稿日期:2022-07-29
  • 最后修改日期:2022-11-28
  • 在线发布日期: 2023-04-06

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